Analysis Note

ControlU251 cell line Skin (Basement Membrane)

Application

Western blot: 1-2µg/mL for detection in whole cell lysate. The antibody is effective against reduced (MW = 130kDa) and non-reduced (MW = 110kDa) proteins.

Immunoprecipitation

Optimal working dilutions must be determined by the end user.

Detect Integrin β1 using this Anti-Integrin β1 Antibody, cytosolic validated for use in IP & WB.

Immunogen

Epitope: cytosolic

Synthetic peptide corresponding to the C-terminus of human integrin beta 1 (a.a. CTTVVNPKYEGK)

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Protein A Purified immunoglobulin. Liquid in PBS containing 0.1% sodium azide.

Format: Purified

Specificity

Reactive with human, mouse, and rat integrin beta-1, as confirmed by western blot. Not reactive with other integrins.

Target description

130kDa